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Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Thermo Fisher bis tris gels nupage mops sds running buffer
Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Santa Cruz Biotechnology nupage
Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Bio-Rad gradient gels nupage
Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% <t>NuPAGE</t> Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.
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Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% NuPAGE Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.

Journal: The Journal of Biological Chemistry

Article Title: Lysyl Hydroxylase 3 Glucosylates Galactosylhydroxylysine Residues in Type I Collagen in Osteoblast Culture *

doi: 10.1074/jbc.M110.178509

Figure Lengend Snippet: Characterization of recombinant LH3-V5/His-tagged protein. Purified LH3-V5/His protein was subjected to a 4–12% NuPAGE Bis-Tris gel, staining with CBB, and Western blot (WB) analyses with anti-V5 antibody (V5), anti-LH3 antibody (LH3), and anti-LH3 antibody with a 15-fold molar excess of blocking peptide (LH3+PB). The major band at the level of ∼96 kDa showed immunoreactivities to both anti-V5 and anti-LH3 antibodies.

Article Snippet: The protein concentration was measured using a DC protein assay kit (Bio-Rad), and the purity of the purified LH3-V5/His protein was determined by a 4–12% NuPAGE Bis-Tris gel and CBB staining.

Techniques: Recombinant, Purification, Staining, Western Blot, Blocking Assay